The church and the adjoining Franciscan convent, rise outside the town of Assisi, in a southerly direction.
The façade of the church is incorporated into a larger, hut, which also includes the rooms adjacent to it. The entrance is preceded by a low porch with three round arches supported by brick pillars; above the central arch there is a circle rose window.
The interior of the church has a single nave covered with an ogival barrel vault, with a rectangular side chapel halfway up the right wall with, on the altar, a valuable wooden crucifix, the work of Innocent of Petralia who signed it in 1637. At the bottom of the nave, there is the deep apse, with a lower vault than that of the classroom; it contains the modern stone high altar, the baroque wooden tabernacle and the monks' choir. In the catino, there is a 14th-century fresco depicting the Madonna and Child between Saints Rufinus and Damian. On the apse, on the other hand, there is a copy of the Crucifix of San Damiano (the original is kept in the basilica of Santa Chiara).
The pipe organ of the church was built in 1957 by the organ company Tamburini di Crema, restored by the balbiani vegezzi-bossi company and equipped with a new console in 1989. With electric transmission, it has 17 registers on two manuals and pedal.
Monday: 10:30 AM – 12:00 AM, 02:00 PM – 06:00 PM
Tuesday: 10:30 AM – 12:00 AM, 02:00 PM – 06:00 PM
Wednesday: 10:30 AM – 12:00 AM, 02:00 PM – 06:00 PM
Thursday: 10:30 AM – 12:00 AM, 02:00 PM – 06:00 PM
Friday: 10:30 AM – 12:00 AM, 02:00 PM – 06:00 PM
Saturday: 10:30 AM – 12:00 AM, 02:00 PM – 06:00 PM
Sunday: 10:30 AM – 12:00 AM, 02:00 PM – 06:00 PM